1.(Biol.) To make a clone from; to make identical copies of an organism by a non-sexual process of reproduction.
2.(Microbiol.) To grow colonies of a microorganism by spreading a suspension of the microorganism onto a solid growth medium (such as in a Petri dish), at a concentration such that individual colonies will grow from single cells sufficiently well separated from other colonies so that pure cultures derived from a single organism can be isolated.
3.(Biochem.) To make large quantities of a segment of DNA by inserting it, using biochemical techniques, into the DNA of a microorganism, and growing that microorganism in large numbers; as, to clone the gene for growth hormone.
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